HPTLC-Based Analysis of Root-Knot Nematode-Induced Changes in L-Cysteine and L-Methionine in Pigeon Pea
HPTLC Analysis of Amino Acids in Pigeon Pea
DOI:
https://doi.org/10.21921/jas.v13i03.15295Keywords:
HPTLC, Pigeonpea, root knot nematode, aminoacidAbstract
Root knot nematode infection causes significant change in the biology and physiology of host plants. In this context, an experiment was conducted to assess change in free amino acids in pigeon pea roots infected by root knot nematode. In HPTLC analysis it was found that L-glutamic acid was seen in all treatments of resistant variety; however, L-ornithine and L-tyrosine were seen in susceptible variety. L-methionine and L-cysteine were absent in resistant healthy varieties, but inoculation of nematodes with these two amino acids appeared. The resistant variety (UPAS-120) infected sample showed a considerable rise in the L-methionine. The amount of the amino acid L-proline was increased most in the roots of the susceptible pigeon pea varieties (IPA-14-7 and CO-6). This method was found to be highly effective and useful for the analysis of amino acids for better understanding of nematode-plant interactions.
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