Management of collar rot in chickpea by Trichoderma species
Management of collar rot disease in chickpea
Keywords:
Trichoderma spp., dual culture, food poisoned technique, parasitizationAbstract
Nine isolates of Trichoderma spp. were isolated from different agro- ecological regions of Nepal viz; Jumla, Palpa, Chitwan, Tarahara, Banke, Illam and Salyan and screened against Sclerotium rolfsii Sacc. Adreded soil borne phytopathogen causing collar rot of chickpea in chickpea; In-vitro efficacy of nine fungal antagonist (Trichoderma spp.) against Sclerotium rolfsii were screened. Pot experiment was done to find out the effective management of S. rolfsi through Tricoderma using different methods i.e. Seed treatment, soil drenching and soil application. All the tested isolates of Trichoderma spp. were found effective on mycelial growth inhibition and sclerotial parasitization of S. rolfsii. Trichoderma isolated from Palpa district showed maximum growth inhibition (%) of pathogen periodically after 48(93.78%), 72(96.00%), 96(97.96%) and 120(100.00%) hours of inoculation. Parasitized sclerotium showed minimum sclerotial germination on agar plates. Moreover, Trichoderma species isolated from Palpa districts showed second best percent mycelial growth inhibition periodically at 72(25.00%), 120(29.16%), 168(29.16%) and 216(29.16%).In pot experiment at 40 days after sowing, Seedling height was maximum in soil drenching with 30g per 100ml of water (22.27cm) and Mortality percentage of seedlings was least or highest disease control was observed in seed treated with 109cfu/ml (0.000%).
References
Ahsan MS, Kumar M and Upadhya JP. 2018. Integrated Approach for the Management of Collar Rot of Chickpea. Int. J. Curr. Microbiol. App. Sci. 7(5): 3560-3569.
Ahsan MS, Kumar M, Upadhyay JP, Minnatullah M and Singh. 2018. Identification of potential isolates of Trichoderma antagonist to sclerotiumrolfsii in the garden soil of litchi and mango orchard. Journal of Entomology and Zoology studies 6(4): 383-388.
Amber P, Akram Abida, Qureshi R and Akram Zaid. 2012. HPLC analysis for secondary metabolites detection in Sclerotiu mrolfsii isolated from chickpea. Pak. J. Bot. 44(2): 417-422.
Anees M, Tronsmo A, Edel-Hermann V, Hjeljord LG, Héraud C and Steinberg C. 2010. Characterization of field isolates of Trichoderma antagonistic against Rhizoctonia solani. Fungal biology 114(9): 691-701.
De Mendiburu F. 2014. Agricolae: statistical procedures for agricultural research. R package version 1(1). https://CRAN.R-project.org/package=agricolae
Dubey SC, Suresh M and Singh B. 2007. Evaluation of Trichoderma species against Fusarium oxysporum f. sp. ciceris for integrated management of chickpea wilt. Biological control 40(1): 118-127.
Eziashi EI, Uma NU, Adekunle AA and Airede CE. 2006. Effect of metabolites produced by Trichoderma species against Ceratocystis paradoxa in culture medium. African Journal of Biotechnology 5: 703-706.
Jana SC and Mandal M. 2017. Antagonistic effect of Trichoderma isolates on Sclerotium rolfsii. Journal of experimental Biology and Agriculture Science 5(4): 506-514.
Jegathambigai V, Wijeratnam RW and Wijesundera RLC. 2010. Effect of Trichoderma spp. on Sclerotium rolfsii, the Causative Agent of Collar Rot on Zamioculcasza miifolia and an on-Farm Method to Mass Produce Trichoderma species. Plant Pathology Journal 9(2): 47-55.
Kumari A and Ghatak A. 2018. Variability in chickpea rot-causing soil-borne necrotrophs, Sclerotium rolfsii and Macrophomina phaseolina. Journal of AgriSearch 5 (4): 247-253.
Rajput VA, Konde SA and Thakur MR. 2010. Evaluation of bioagents against chickpea wilt complex. Journal of Soils and Crops 20(1): 155-158.
Singh AK, Singh SS, Prakash V, Kumar S and Dwivedi SK. 2015. Pulses Production in India: Present Status, Bottleneck and Way Forward. Journal of AgriSearch 2 (2): 75-83.
Singh D, Kumar A, Singh AK and Tripathi HS. 2013. Severity of chickpea wilt in north Bihar and nutritional studies on fusarium oxysporum f.sp. Ciceri J. Plant Disease Sci. 8(2):137-140.
Wavare SH, Gade, RM and Shitole AV. 2017. Effect of plant extracts, bio agents and fungicides against Sclerotium rolfsii causing collar rot in chickpea. Indian Journal of Pharmaceutical Sciences 79(4): 513-520.
