An experiment was carried out at instructional cum research farm, Department of Horticulture, BNCA, AAU, Biswanath Chariali during 2018-2019 to standardize the propagation method of custard apple (Annona reticulata) by air layering. The experiment was laid out in a factorial completely randomized design with four treatments viz., pre-conditioned with 30 days of girdling (T₁), pre-conditioned with 30 days of etiolation (T₂), pre-conditioned with 30 days of girdling + etiolation (T₃) and control i.e. air layering without pre-conditioning (T₄) with three replications and all these treatments were applied at monthly interval from March to August, 2018. Among the treatments, T₂ recorded the highest percentage of success of layering (62.37%), required minimum number of days for separation of layers from mother plant (53.02 days), maximum numbers of primary roots per layer (4.76), percentage of survivability (89.05%) and number of new shoots per layer at 30,60, 90 days after planting i.e., 2.21, 2.78 and 3.09. On an average, air layering done in August (S₆) produced highest percentage of success (61.44%), minimum days for separation of layers from mother plant (51.40), numbers of primary roots per layer (5.35), numbers of new shoots per layer at 30 days (2.27), 60 days (2.75) and at 90 days (3.12) after planting in poly bags.