Acacia nilotica (L.) commonly known as babul is a multipurpose nitrogen fixing tree which provides timber, fuel, shade, food, fodder, dye and gum. Seed maturity indices were studied and seeds collected during 2^nd week of June (5^th collection) resulted maximum germination (85.2+0.46 %). At this stage, the colour of pod and seed was yellowish white and brownish black, respectively. In ordinary storage conditions, seeds get damaged due to insect pest attacks. Hence, a study was conducted to standardize the optimum seed storage conditions for getting maximum germination. The seeds were stored up to 600days in different containers at different temperatures viz. poly bag at room temperature 15-40⁰C (B₁T₁), poly bag at BOD25⁰C (B₁T₂), poly bag at refrigerator 5⁰C (B₁T₃), paper bag at room temperature 15-40⁰ C (B₂T₁), paper bag at BOD 25⁰C (B₂T₂), paper bag at refrigerator 5⁰ C (B₂T₃), cloth bag at room temperature 15-40⁰ C (B₃T₁), cloth bag at BOD 25⁰C (B₃T₂) and cloth bag at refrigerator 5⁰ C (B₃T₃). The results revealed that seeds stored for 15 days in poly bag at refrigerator 5⁰C(B₁T₃) recorded the maximum germination of 85.2 % and 81.12 % in laboratory and field conditions, respectively. The seed germination decreased with advancement of storage period in all the storage treatments. However, after 600 days of storage period, the maximum seed germination (59.1%) was recorded in B₁T₃in laboratory conditions_. The seeds stored in poly bags as well as in cloth bags and stored in refrigerator gave maximum germination in laboratory and field conditions. Hence, it may be concluded that the seeds of A. nilotica can be stored either in poly bag or in cloth bag in refrigerator at 5⁰ C for long term storage to get maximum germination.