Genetic diversity analysis among finger millet genotypes using simple sequence repeat markers
SSR-based diversity in finger millet genotypes
DOI:
https://doi.org/10.21921/jas.v12i04.15256Keywords:
Finger millet, SSR, DNA isolation, PolymorphismAbstract
Finger millet is a nutritionally rich, climate-resilient cereal crop with significant genetic variability. Understanding its genetic diversity is essential for effective conservation, improvement, and breeding programs. In this study, 4 simple sequence repeat markers were used to assess the genetic diversity among 7 finger millet genotypes. Microsatellites based genetic similarity between IE2051 and RAU-8 (0.7500) and IE2253 and IE2252 (0.7500) was found to be the maximum amongst pair-wise combinations of the entries. Cluster analysis using the UPGMA method grouped the 7 genotypes into 2 major clusters, and further into subclusters, suggesting clear genetic relationships among finger millet genotypes. The findings of this study highlight the effectiveness of SSR markers in detecting genetic variability and identifying genetically diverse genotypes. This result can be utilized for breeding strategies, germplasm conservation, and genetic enhancement of finger millets. SSR markers effectively revealed significant genetic diversity among finger millet genotypes. SSR markers proved to be highly informative and effective for detecting polymorphism among finger millet genotypes. The result will contribute to the breeding program for improving finger millet genotypes.
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